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KMID : 1161920140110010001
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Journal of Medicine and Life Science
2014 Volume.11 No. 1 p.1 ~ p.4
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Establishment of Assay Model for the Trophoblast Cell-line Proliferation
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Shim Soon-Sup
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Abstract
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Preeclampsia is one of the major causes of pregnancy-related mortality and morbidity. While the pathophysiology is not well examined, it is known that impeded early placental formation may be one etiologic step. The cytotoxic effect of lymphocytes on the trophoblast may be one of immunological explanations. This study was designed to find out the optimal condition of cell concentration and to establish an experimental model of cytotoxic effect of lymphocytes on the trophoblast cell-line proliferation. In various conditions of cell concentration, the interaction between trophoblast cell-line and lymphocytes were measured by CCK-8 kit, which assesses cell proliferation by absortiometry. The optimal condition (trophoblast cell-line: 105 cells/mL, lymphocyte: 106 cells/mL) was selected for this model. We established a model for assessing the interaction between the trophoblast cell-line and lymphocytes by measuring the cell proliferation.
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KEYWORD
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Preeclampsia, Experimental Model, Trophoblast Cell-line, Lymphocytes, Cell Proliferation
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